The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken

The replacement histone H2A.Z is variously reported as being linked to gene expression and preventing the spread of heterochromatin in yeast, or concentrated at heterochromatin in mammals. To resolve this apparent dichotomy, affinity-purified antibodies against the N-terminal region of H2A.Z, in both a triacetylatedandnon- acetylatedstate, areusedin native chromatin immmuno-precipitation experiments with mononucleosomes from three chicken cell types. The hyperacetylated species concentrates at the 50 end of active genes, both tissue specific and housekeeping but is absent from inactive genes, while the unacetylated form is absent from both active and inactive genes. A concentration of H2A.Z is also found at insulators under circumstances implying a link to barrier activity but not to enhancer blocking. Although acetylated H2A.Z is widespread throughout the interphase genome, at mitosis its acetylation is erased, the unmodified form remaining. Thus, although H2A.Z may operate as an epigenetic marker for active genes, its N-terminal acetylation does not

The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken

The replacement histone H2A.Z is variously reported as being linked to gene expression and preventing the spread of heterochromatin in yeast, or concentrated at heterochromatin in mammals. To resolve this apparent dichotomy, affinity-purified antibodies against the N-terminal region of H2A.Z, in both a triacetylatedandnon- acetylatedstate, areusedin native chromatin immmuno-precipitation experiments with mononucleosomes from three chicken cell types. The hyperacetylated species concentrates at the 50 end of active genes, both tissue specific and housekeeping but is absent from inactive genes, while the unacetylated form is absent from both active and inactive genes. A concentration of H2A.Z is also found at insulators under circumstances implying a link to barrier activity but not to enhancer blocking. Although acetylated H2A.Z is widespread throughout the interphase genome, at mitosis its acetylation is erased, the unmodified form remaining. Thus, although H2A.Z may operate as an epigenetic marker for active genes, its N-terminal acetylation does not

Sub-millimeter images of a dusty Kuiper belt around eta Corvi

We present sub-millimeter and mid-infrared images of the circumstellar disk around the nearby F2V star eta Corvi. The disk is resolved at 850um with a size of ~100AU. At 450um the emission is found to be extended at all position angles, with significant elongation along a position angle of 130+-10deg; at the highest resolution (9.3") this emission is resolved into two peaks which are to within the uncertainties offset symmetrically from the star at 100AU projected separation. Modeling the appearance of emission from a narrow ring in the sub-mm images shows the observed structure cannot be caused by an edge-on or face-on axisymmetric ring; the observations are consistent with a ring of radius 150+-20AU seen at 45+-25deg inclination. More face-on orientations are possible if the dust distribution includes two clumps similar to Vega; we show how such a clumpy structure could arise from the migration over 25Myr of a Neptune mass planet from 80-105AU. The inner 100AU of the system appears relatively empty of sub-mm emitting dust, indicating that this region may have been cleared by the formation of planets, but the disk emission spectrum shows that IRAS detected an additional hot component with a characteristic temperature of 370+-60K (implying a distance of 1-2AU). At 11.9um we found the emission to be unresolved with no background sources which could be contaminating the fluxes measured by IRAS. The age of this star is estimated to be ~1Gyr. It is very unusual for such an old main sequence star to exhibit significant mid-IR emission. The proximity of this source makes it a perfect candidate for further study from optical to mm wavelengths to determine the distribution of its dust.Comment: 22 pages, 4 figures. Scheduled for publication in ApJ 10 February 2005 issu

Oral contraceptives, hormone replacement therapy, thrombophilias and risk of venous thromboembolism: a systematic review The Thrombosis: Risk and Economic Assessment of Thrombophilia Screening (TREATS) Study

Combined oral contraceptives,oral hormone replacement therapy and thrombophilias are recognised risk factors for venous thromboembolism in women.The objective of this study was to assess the risk of thromboembolism among women with thrombophilia who are taking oral contraceptives or hormone replacement therapy, conducting a systematic review and metaanalysis. Of 201 studies identified, only nine met the inclusion criteria. Seven studies included pre-menopausal women on oral contraceptives and two studies included peri-menopausal women on hormone replacement therapy. For oral contraceptive use, significant associations of the risk of venous thromboembolism were found in women with factor V Leiden (OR 15.62; 95%CI 8.66 to 28.15); deficiencies of antithrombin (OR 12.60; 95%CI 1.37 to 115.79), protein C (OR 6.33; 95%CI 1.68 to 23.87), or protein S (OR 4.88; 95%CI 1.39 to 17.10), elevated levels of factor VIIIc (OR 8.80; 95%CI 4.13 to 18.75); and factor V Leiden and prothrombin G20210A (OR 7.85; 95%CI 1.65 to 37.41). For hormone replacement therapy, a significant association was found in women with factor V Leiden (OR 13.16; 95%CI 4.28 to 40.47).Although limited by the small number of studies, the findings of this study support the presence of interaction between thrombophilia and venous thromboembolism among women taking oral contraceptives. However, further studies are required to establish with greater confidence the associations of these, and other, thrombophilias with venous thromboembolism among hormone users

CO emission from discs around isolated HAeBe and Vega-excess stars

We describe results from a survey for J=3-2 12CO emission from visible stars with an infrared excess. The line is clearly detected in 21 objects, with molecular gas (>10^-3 Jupiter masses) common in targets with infrared excesses >0.01 (>56% of objects). Such high excesses indicate the presence of a disc of opening angle >12 degrees; within this, the optically thick disc prevents CO photodissociation. Two or three stars with associated CO have an excess <0.01, implying a disc opening angle <1 degree. Most line profiles are double-peaked or relatively broad. Model fits, assuming a Keplerian disc, indicate outer radii, R_out, of ~20-300 au. As many as 5 discs have outer radii smaller than the Solar System (50 au), and a further 4 have gas at radii <20 au. R_out is independent of the stellar spectral type (from K through to B9), but is correlated with total dust mass. R_out appears to decrease with time: discs around stars of age 3-7 Myr have a mean radius of ~210 au, whereas discs of age 7-20 Myr are a factor of 3 smaller. The only bona fide debris disc with detected CO is HD9672; this has a double peaked line profile and is the most compact gas disc observed, with a modelled radius 17 au). A fit to HD141569 suggests the gas lies in two rings of radii 90 and 250 au, similar to the scattered light structure. In both AB Aur and HD163296 the sizes of the molecular and dust scattering discs are also similar, suggesting that the gas and small dust grains are co-located.Comment: 16 pages, 5 figures MNRAS - accepte

Outpatient management of biliary colic: A prospective observational study of prescribing habits and analgesia effectiveness

AbstractBackgroundUncomplicated biliary colic presents a significant health and financial burden to hospitals and primary care services alike. There is little guidance on the correct analgesia to use on an outpatient basis. This study aimed to evaluate the effectiveness of oral analgesics on biliary colic pain and to explore the prescribing habits of community doctors.MethodsConsecutive patients with ultrasound proven symptomatic gallstones completed a questionnaire recording demographics and symptomatology. Pain was assessed using a visual analogue scale (VAS) based on the Biliary Symptom Score (BSS) to evaluate the effectiveness of various analgesic agents. Local General Practitioners were also surveyed to establish prescribing practices.ResultsCo-Codamol had the highest mean effectiveness VAS score (6.5/10). Patients with increased BMI, short symptom duration and a BSS >70 were most likely to suffer from severe pain. Patients in a subgroup with severe pain were most likely to have their pain reduced by NSAID analgesia compared to no NSAID (OR 2.20, p = 0.027). This effect remained significant upon multivariable regression (OR 2.52, p = 0.018) in a model containing age and NSAIDs. There was wide variation in the prescribing practice of GPs and hospital doctors.ConclusionsThe range of drugs prescribed for biliary colic is extensive with little evidence base. In this study NSAIDs were the most effective analgesia for patients with severe pain. In the absence of contraindications to their use, physician education or guidance emphasizing the benefits of NSAIDs may potentially reduce symptomatic hospital presentation and admissions for biliary colic

Relationship between Diffusion, Selfdiffusion and Viscosity

We investigate the experimental limits of validity of the Stokes-Einstein equation. There is an important difference between diffusion and self-diffusion. There are experimental evidences, that in the case of self-diffusion the product D /T is constant and the equation is still valid. However, comparison of existing experimental data on viscosity and diffusion coefficients D of small, fast moving ions unambiguously show that the product D /T depends strongly on temperature T. The temperature dependence of diffusion coefficient declines from that of viscosity. Therefore, the Stokes-Einstein equation is not valid in this case

Auxetic regions in large deformations of periodic frameworks

In materials science, auxetic behavior refers to lateral widening upon stretching. We investigate the problem of finding domains of auxeticity in global deformation spaces of periodic frameworks. Case studies include planar periodic mechanisms constructed from quadrilaterals with diagonals as periods and other frameworks with two vertex orbits. We relate several geometric and kinematic descriptions.Comment: Presented at the International Conference on "Interdisciplinary Applications of Kinematics" (IAK18), Lima, Peru, March 201